Composite

Part:BBa_K3734032:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-11)


9XUAS-Ad-LUC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 301
    Illegal NgoMIV site found at 1645
    Illegal NgoMIV site found at 1666
    Illegal AgeI site found at 1369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1551


Design Notes

We use 9XUAS.When you use PCR to clone the fragment, you need to focus on how many times the UAS repeats.


Source

UAS is from yeast cell. LUC is from glowworm

References