Composite
Part:BBa_K3734032:Design
Designed by: Xingjun Zhao Group: iGEM21_CSU_CHINA (2021-10-11)
9XUAS-Ad-LUC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 38
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 301
Illegal NgoMIV site found at 1645
Illegal NgoMIV site found at 1666
Illegal AgeI site found at 1369 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1551
Design Notes
We use 9XUAS.When you use PCR to clone the fragment, you need to focus on how many times the UAS repeats.
Source
UAS is from yeast cell. LUC is from glowworm